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A silicone pad placed inside a 150 mm x 20 mm deep plastic petri dish. The mat can be removed, washed, and returned to the dish for reuse. The pins come in 10 different coded colors. An optional Pyrex Glass Petri dish is available for use when sterilization is needed

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End date: May 21, 2021

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4. Put the small Petri dish containing the tissue holder into a large Petri dish and put the large Petri dish inside the vacuum chamber. Follow these instructions for each step of the processing. 5. Seal the lid tightly for each step of the processing for which there is a vacuum cycle (refer to Table 3). 6.

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7. Pour the molten agar into the open Petri dishes, using 8 ml agar for a 5-cm dish and 25 ml for a 10-cm one. Coat standard microscope slides to a depth of 1 to 2 mm, pouring 3 to 5 ml of the molten agar over the slides. The task is easier if the slides are held in a plastic mould on a levelling table. 8.

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Mar 31, 2020 · Using a plastic paraffin mold and glass pipet, gently transfer one embryo to the mold. Carefully remove PBST from embryo. Place the embryo in a sagittal position. Quickly, add about 0.5 mL of hot agarose to the mold - just enough to cover the embryo and fill the mold. Ensure that no air bubbles surround the embryo.

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